《病毒學方法雜志》發表了關于病毒學各方面技術的原創論文和特邀評論。這些方法包括研究病毒及其成分的形態、組裝、復制、成分、功能和理化特性；病毒及其成分的純化、培養；病毒抗原的性質，抗體的產生；研究對病毒、病毒亞基和成分的免疫反應的技術；病毒和病毒感染的檢測和鑒定；病毒和病毒傳染性的測定以及傳播和致病性的調查；以及研究抑制或抑制病毒生長的方法。

The Journal of Virological Methods publishes original papers and invited reviews covering techniques on all aspects of virology. These include methods for studying the morphology, assembly, replication, composition, function and physiochemical properties of viruses and their components; the purification of viruses and their components; cultivation; properties of viral antigens, production of antibody, and techniques for studying the immune response to virions, viral subunits, and components; the detection and identification of viruses and viral infections; assay of viruses and viral infectivity and the investigation of transmission and pathogenicity; and methods for investigating the suppression or inhibition of viral growth.